Overview
Become qualified to collect urine specimens for DOT-regulated drug tests. This online course covers the qualification training required by 49 CFR §40.33, followed by a 48-question final assessment. To finish qualifying, you complete five consecutive error-free mock collections.
7 modules~66 min narrated
48 questionsfinal assessment
80%to pass, unlimited retakes
5 mocksproficiency demo
Requalify every 5 years. DOT requires urine specimen collectors to complete refresher training at least every five years (49 CFR §40.33(d)), including five new error-free mock collections. This course works for both initial qualification and refresher training.
Curriculum
Module 1: Foundations, Roles, and Collector Qualification~13.5 min
Module 2: Collection Sites, Security, Supplies, and the 2026 CCF~9.5 min
Module 3: Routine Collection I: Arrival Through Specimen Receipt~11 min
Module 4: Routine Collection II: Split, Seal, Complete, and Ship~7 min
Module 5: Insufficient Specimen and Unusual Collection Situations~7.5 min
Module 6: Monitored, Directly Observed, Temperature, and Tampering~9 min
Module 7: Corrections, Fatal and Correctable Flaws, Records, and Course Completion~9 min
Final assessment: 48 questions, 80% to passunlimited retakes
Course outline
Every module and lesson in the course. Click a module to expand it.
Module 1: Foundations, Roles, and Collector Qualification
- Purpose of DOT specimen collection
- How Part 40, the Guidelines, and agency rules fit together
- Key federal regulations and agencies
- FTA covered functions
- DOT Regulatory Authorities
- DOT Safety Priorities
- The six reasons for DOT testing and the industries covered
- Who does what: your working relationships
- Collector responsibilities and compliance basics
- Becoming qualified: training and five mock collections (§40.33)
- Staying qualified: refresher and error-correction training
- Who may not collect, and professional conduct (§40.31)
- Your records, the employee's privacy, and specimen integrity
✓ Module 1 knowledge check
Module 2: Collection Sites, Security, Supplies, and the 2026 CCF
- What makes a collection site acceptable (§40.42)
- Preparing single-stall and multi-stall restrooms
- Securing water, removing adulterants, and controlling access (§40.43)
- Required supplies: kits, seals, and shipping materials
- Understanding the 2026 Federal CCF
- Verify the information before you begin
✓ Module 2 knowledge check
Module 3: Routine Collection I: Arrival Through Specimen Receipt
- Beginning promptly (§40.61(a)–(b))
- Verifying the employee's identity (§40.61(c)–(d))
- Confirming testing instructions and explaining the process
- Preparing the employee: clothing, belongings, and pockets (§40.61(f))
- When you find something suspicious (§40.61(f)(5))
- Clear instructions while protecting privacy (§40.63)
- Receiving the specimen: the three immediate checks (§40.65)
- When a check fails: insufficient, out-of-range, or tampered
✓ Module 3 knowledge check
Module 4: Routine Collection II: Split, Seal, Complete, and Ship
- Splitting the specimen: Bottle A and Bottle B (§40.71)
- Applying seals, dating, and employee initials
- Damaged, reversed, or improperly applied seals
- Completing the CCF: Step 5 (employee) and Step 4 (collector)
- Distributing the five CCF copies
- Packaging, storing, and shipping with an unbroken chain of custody
- Electronic CCF (eCCF) procedures
✓ Module 4 knowledge check
Module 5: Insufficient Specimen and Unusual Collection Situations
- Recognizing an insufficient specimen and starting the three-hour clock
- Fluids, attempts, supervision, and never combining urine
- When the employee can't provide a sufficient specimen in time
- Inability or refusal? Knowing the difference
- Unusual medical situations and privacy accommodations
✓ Module 5 knowledge check
Module 6: Monitored, Directly Observed, Temperature, and Tampering
- Monitored vs. directly observed collections
- When direct observation is required
- Who may observe or monitor: same-sex requirement and exceptions
- Performing a directly observed collection with dignity
- Temperature, unusual characteristics, and suspected substitution or adulteration
- The second, directly observed collection and linking two CCFs
- Refusals, observation problems, and communicating with the DER
✓ Module 6 knowledge check
Module 7: Corrections, Fatal and Correctable Flaws, Records, and Course Completion
- The seven fatal flaws
- Fatal flaws vs. correctable flaws vs. other errors
- Correcting CCF errors: signed statements and memoranda
- When error-correction training is required
- Records, confidentiality, and keeping your qualification file
- DOT Audits and Undercover Reviews
- Putting it all together: full procedure review
- Completing the course: final assessment and five mock collections
✓ Module 7 knowledge check
Final Assessment
✓ Final assessment (48 questions, 80% to pass)
Your Certificate
When you complete the course and pass the final assessment, you receive a downloadable certificate of completion in your name. It documents completion of the knowledge portion of qualification training; the five mock collections required by 49 CFR §40.33(c) are completed separately.
Printable resource forms included
Every enrollment includes downloadable PDF forms to use while you study and on the job:
- Collection checklist
- Shy bladder log
- Sample CCF walkthrough (annotated)
- Full-page sample Federal CCF (all 5 copies)
- Direct observation checklist
- Problem collections quick reference
- Collector qualification and mock collections tracker
What's included
| Item | Included |
|---|
| Online qualification training (7 modules) | Yes |
| Final assessment, unlimited retakes | Yes |
| Printable resource forms (7 PDFs) | Yes |
| 5 mock collections | Available separately, $129. After you enroll, you'll receive a link to book your live online mock sessions at a time that works for you. Sessions are held live online with a Get Certable monitor. |
| Collection supplies for mocks | Included with your mock collections. Get Certable ships you the collection supplies you need for your mocks. |
| Certificate of completion | Yes (see sample below) |
Who this course is for
- New collectors who need qualification training
- Current collectors due for refresher training
- Clinics and testing businesses training staff
Employer & group registration
Training several collectors? We offer group pricing for employers, clinics and testing businesses. Email support@getcertable.com with the number of people and we'll send a quote.
